Synthesis, and biological screening of chloropyrazine conjugated benzothiazepine derivatives as potential antimicrobial, antitubercular and cytotoxic agents

A series of twenty new chloropyrazine conjugated benzothiazepines (22–41) have been synthesized with 58%–95% yields. The compounds were characterized by using different spectroscopic techniques including FT-IR, 1H NMR, 13C NMR spectroscopy and mass spectrometry. their precursor chalcones (2–21) evaluated for antitubercular cytotoxic activities. Additionally, 22–41 also tested antimicrobial activity. Among the chalcone (2–21), 7 14 showed significant activities (MICs 25.51 23.89 µM, respectively), whereas among (22–41), 27 34 displayed 38.02 19.01 µM) (MIC 18.10 Compounds 41 IC50 46.03 ± 1 35.10 2 µM respectively. All activity on normal human liver cell lines (L02) found to be relatively less selective towards this line. most active identified through study could considered as potential leads development drugs possible antimicrobial, antitubercular,